Archaebacterial elongation factor Tu insensitive to pulvomycin and kirromycin
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چکیده
منابع مشابه
Structural investigations of kirromycin bound to bacterial elongation factor Tu by NMR and molecular dynamics.
Kirromycin, also known as mccimycin, is a member of the elfamycin family of antibiotics. These antibiotics inhibit bacterial protein synthesis by binding tightly to elongation factor Tu (Emu) a 43.2 kD G-protein. E m u .GTP is able to carry aminoacyl tRNA to the ribosome in the presence of kirromycin in the usual way, but after hydrolysis of GTP EFTu.GDP is unable to leave the ribosome. Consequ...
متن کاملThe unique tuf2 gene from the kirromycin producer Streptomyces ramocissimus encodes a minor and kirromycin-sensitive elongation factor Tu.
Streptomyces ramocissimus, the producer of elongation factor Tu (EF-Tu)-targeted antibiotic kirromycin, contains three divergent tuf-like genes, with tuf1 encoding regular kirromycin-sensitive EF-Tu1; the functions of tuf2 and tuf3 are unknown. Analysis of the tuf gene organization in nine producers of kirromycin-type antibiotics revealed that they all contain homologues of tuf1 and sometimes o...
متن کاملThe molecular basis of kirromycin (mocimycin) action; a 1H NMR study using deuterated elongation factor Tu.
The binding of the antibiotic kirromycin (mocimycin) to its target protein, bacterial elongation factor Tu (EF-Tu), has been studied by 1H NMR spectroscopy using deuterated protein. Narrow lines were observed in the spectrum of the unbound protein (due to residual protons) and in the spectrum of the kirromycin-EF-Tu complex. The spectrum of the complex has been compared with the spectra of the ...
متن کاملNatural kirromycin resistance of elongation factor Tu from the kirrothricin producer Streptomyces cinnamoneus.
The antibiotic kirromycin (Kr) inhibits bacterial protein synthesis by binding to elongation factor Tu (EF-Tu). Streptomyces cinnamoneus and Nocardia lactamdurans, producers of antibiotics of the Kr class, are known to possess an EF-Tu resistant to Kr. Both micro-organisms appear to possess a single tuf gene and we have characterized the one from S. cinnamoneus, which belongs to the tuf1 family...
متن کاملKirromycin, an inhibitor of protein biosynthesis that acts on elongation factor Tu.
Kirromycin, a new inhibitor of protein synthesis, is shown to interfere with the peptide transfer reaction by acting on elongation factor Tu (EF-Tu). All the reactions associated with this elongation factor are affected. Formation of the EF-Tu.GTP complex is strongly stimulated. Peptide bond formation is prevented only when Phe-tRNA(Phe) is bound enzymatically to ribosomes, presumably because G...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1982
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)80819-3